Review



rch acv  (DSMZ)


Bioz Verified Symbol DSMZ is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    DSMZ rch acv
    a , Menin ChIP-seq after 24 h treatment with DMSO or 250 nM Menin inhibitor VTP50469 (MENi) in <t>SEM,</t> <t>RCH-ACV</t> and OCI-AML3 cells. Metaplots show mean reference normalized reads per million (RRPM) centered on transcription start sites (TSS) genome-wide. b , Dose response curves to MENi recorded at 48 h using CellTiterGlo (n = 5 for SEM and RCH-ACV cells, n = 3 for OCI-AML3 cells). c , Colony forming units recorded after culture in Menin inhibitor (n=5). Error bars represent standard error. d , Violin plots depicting the log2 fold changes of differentially expressed genes (FDR < 0.05) after 24 h MENi (250 nM), split by Menin binding at the promoter. e , Upset plot representing the cell lines in which shared Menin-bound genes (centre of Venn diagram in ) are downregulated following MENi (250 nM) for 24 h. f , Intersections of all upregulated genes following 24 h MENi (250 nM) in SEM, OCI-AML3, and RCH-ACV cells. g , Correlation heatmap of genome-wide Menin ChIP-seq signal in cell lines (SEM, RCH-ACV, and OCI-AML3) and Menin ChIPmentation in primary patient samples (NPM1c-AML, iALL28349, iALL863388). h , Heatmaps and metaplots showing the mean Menin signal at promoters in SEM cells (ChIP-seq) and two primary patient samples (ChIPmentation), sorted and k-means clustered by Menin signal in SEM cells. i , Heatmaps and metaplots showing the mean Menin signal at promoters in OCI-AML3 cells (ChIP-seq) and one primary patient sample (ChIPmentation), clustered by Menin signal in OCI-AML3 cells.
    Rch Acv, supplied by DSMZ, used in various techniques. Bioz Stars score: 96/100, based on 504 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rch+acv/bio_rxiv__64898__2026__01__16__698179-208-0-3?v=DSMZ
    Average 96 stars, based on 504 article reviews
    rch acv - by Bioz Stars, 2026-07
    96/100 stars

    Images

    1) Product Images from "Menin maintains enhancer-promoter interactions in a leukemia-specific manner"

    Article Title: Menin maintains enhancer-promoter interactions in a leukemia-specific manner

    Journal: bioRxiv

    doi: 10.64898/2026.01.16.698179

    a , Menin ChIP-seq after 24 h treatment with DMSO or 250 nM Menin inhibitor VTP50469 (MENi) in SEM, RCH-ACV and OCI-AML3 cells. Metaplots show mean reference normalized reads per million (RRPM) centered on transcription start sites (TSS) genome-wide. b , Dose response curves to MENi recorded at 48 h using CellTiterGlo (n = 5 for SEM and RCH-ACV cells, n = 3 for OCI-AML3 cells). c , Colony forming units recorded after culture in Menin inhibitor (n=5). Error bars represent standard error. d , Violin plots depicting the log2 fold changes of differentially expressed genes (FDR < 0.05) after 24 h MENi (250 nM), split by Menin binding at the promoter. e , Upset plot representing the cell lines in which shared Menin-bound genes (centre of Venn diagram in ) are downregulated following MENi (250 nM) for 24 h. f , Intersections of all upregulated genes following 24 h MENi (250 nM) in SEM, OCI-AML3, and RCH-ACV cells. g , Correlation heatmap of genome-wide Menin ChIP-seq signal in cell lines (SEM, RCH-ACV, and OCI-AML3) and Menin ChIPmentation in primary patient samples (NPM1c-AML, iALL28349, iALL863388). h , Heatmaps and metaplots showing the mean Menin signal at promoters in SEM cells (ChIP-seq) and two primary patient samples (ChIPmentation), sorted and k-means clustered by Menin signal in SEM cells. i , Heatmaps and metaplots showing the mean Menin signal at promoters in OCI-AML3 cells (ChIP-seq) and one primary patient sample (ChIPmentation), clustered by Menin signal in OCI-AML3 cells.
    Figure Legend Snippet: a , Menin ChIP-seq after 24 h treatment with DMSO or 250 nM Menin inhibitor VTP50469 (MENi) in SEM, RCH-ACV and OCI-AML3 cells. Metaplots show mean reference normalized reads per million (RRPM) centered on transcription start sites (TSS) genome-wide. b , Dose response curves to MENi recorded at 48 h using CellTiterGlo (n = 5 for SEM and RCH-ACV cells, n = 3 for OCI-AML3 cells). c , Colony forming units recorded after culture in Menin inhibitor (n=5). Error bars represent standard error. d , Violin plots depicting the log2 fold changes of differentially expressed genes (FDR < 0.05) after 24 h MENi (250 nM), split by Menin binding at the promoter. e , Upset plot representing the cell lines in which shared Menin-bound genes (centre of Venn diagram in ) are downregulated following MENi (250 nM) for 24 h. f , Intersections of all upregulated genes following 24 h MENi (250 nM) in SEM, OCI-AML3, and RCH-ACV cells. g , Correlation heatmap of genome-wide Menin ChIP-seq signal in cell lines (SEM, RCH-ACV, and OCI-AML3) and Menin ChIPmentation in primary patient samples (NPM1c-AML, iALL28349, iALL863388). h , Heatmaps and metaplots showing the mean Menin signal at promoters in SEM cells (ChIP-seq) and two primary patient samples (ChIPmentation), sorted and k-means clustered by Menin signal in SEM cells. i , Heatmaps and metaplots showing the mean Menin signal at promoters in OCI-AML3 cells (ChIP-seq) and one primary patient sample (ChIPmentation), clustered by Menin signal in OCI-AML3 cells.

    Techniques Used: ChIP-sequencing, Genome Wide, Binding Assay

    a , Volcano plots of transient transcriptome RNA-seq after 24 h of treatment with DMSO or 250 nM Menin inhibitor VTP50469. b , Mean Menin binding (RRPM = reference normalised ChIP-seq reads per million) in SEM, RCH-ACV, and OCI-AML3 cells at promoters of differentially expressed genes in control (DMSO) or Menin inhibitor treatment (MENi). c , Proportion of differentially expressed genes (n.s. = not significant) following MENi with Menin binding at the promoter (± 2 kb around the TSS). Reported p -values are from a Pearson chi-square test. d , Intersections of all Menin-bound genes in the cell line panel. e , Intersections of downregulated Menin target genes in the cell line panel. f , Examples of Menin ChIP-seq in cell lines (SEM, RCH-ACV, and OCI-AML3) and ChIPmentation in primary patient samples (NPM1c-AML, iALL28349, and iALL863388) at down-regulated genes after 24 h MENi in the indicated cell lines.
    Figure Legend Snippet: a , Volcano plots of transient transcriptome RNA-seq after 24 h of treatment with DMSO or 250 nM Menin inhibitor VTP50469. b , Mean Menin binding (RRPM = reference normalised ChIP-seq reads per million) in SEM, RCH-ACV, and OCI-AML3 cells at promoters of differentially expressed genes in control (DMSO) or Menin inhibitor treatment (MENi). c , Proportion of differentially expressed genes (n.s. = not significant) following MENi with Menin binding at the promoter (± 2 kb around the TSS). Reported p -values are from a Pearson chi-square test. d , Intersections of all Menin-bound genes in the cell line panel. e , Intersections of downregulated Menin target genes in the cell line panel. f , Examples of Menin ChIP-seq in cell lines (SEM, RCH-ACV, and OCI-AML3) and ChIPmentation in primary patient samples (NPM1c-AML, iALL28349, and iALL863388) at down-regulated genes after 24 h MENi in the indicated cell lines.

    Techniques Used: RNA Sequencing, Binding Assay, ChIP-sequencing, Control

    a , UpSet plot representing significantly enriched proteins (FDR < 0.01 compared to IgG controls) co-immunoprecipitated with Menin in SEM, OCI-AML3, and RCH-ACV cells. b , Heatmap of statistically enriched Menin interactors in SEM, RCH-ACV and OCI-AML3 cells. c , Global enrichment of select complexes involved in transcription in Menin co-immunoprecipitations. d , Correlation between the gene effect scores reported in DepMap for SEM and OCI-AML3 CRISPR screens. Proteins within complexes specifically enriched in SEM cells are colored. Genes with an effect score of < -1 in both cell lines are labelled.
    Figure Legend Snippet: a , UpSet plot representing significantly enriched proteins (FDR < 0.01 compared to IgG controls) co-immunoprecipitated with Menin in SEM, OCI-AML3, and RCH-ACV cells. b , Heatmap of statistically enriched Menin interactors in SEM, RCH-ACV and OCI-AML3 cells. c , Global enrichment of select complexes involved in transcription in Menin co-immunoprecipitations. d , Correlation between the gene effect scores reported in DepMap for SEM and OCI-AML3 CRISPR screens. Proteins within complexes specifically enriched in SEM cells are colored. Genes with an effect score of < -1 in both cell lines are labelled.

    Techniques Used: Immunoprecipitation, CRISPR

    a , Pearson correlations between nuclear proteome samples (left) and Menin immunoprecipitation (IP) samples (right), computed on log2-transofrmed and imputed data. b , Correlation in the abundance of differentially enriched complex components in the nuclear proteomes of SEM and OCI-AML3 cells. The grey dashed line represents a slope of 1. Pearson R values are shown for each complex. c , Comparison of proteins enriched in Menin IPs in SEM versus RCH-ACV cells (left) and SEM versus OCI-AML3 cells (right). Only proteins that were statistically enriched over IgG controls in at least one cell line (FDR < 0.01) were included.
    Figure Legend Snippet: a , Pearson correlations between nuclear proteome samples (left) and Menin immunoprecipitation (IP) samples (right), computed on log2-transofrmed and imputed data. b , Correlation in the abundance of differentially enriched complex components in the nuclear proteomes of SEM and OCI-AML3 cells. The grey dashed line represents a slope of 1. Pearson R values are shown for each complex. c , Comparison of proteins enriched in Menin IPs in SEM versus RCH-ACV cells (left) and SEM versus OCI-AML3 cells (right). Only proteins that were statistically enriched over IgG controls in at least one cell line (FDR < 0.01) were included.

    Techniques Used: Immunoprecipitation, Comparison

    a , Heatmaps of Menin ChIP-seq at promoters and enhancers in SEM, RCH-ACV, and OCI-AML3 cells. Regions are sorted by H3K27ac signal and centered on ATAC peaks. b , Heatmaps of Menin ChIPmentation at promoters and enhancers, centred on H3K27ac peaks, in primary samples from two infant MLL-AF4 ALL patients (iALL28349, iALL863388) and one adult NPM1-mutated AML patient. c , Association between Menin binding at intragenic enhancers and differential gene expression from the nearest TSS following 24 h of MENi. p -values are reported from a Pearson chi-square test. d , Mean Menin, MLL/MLL-AF4, and H3K27ac at intragenic enhancers annotated to differentially expressed genes by nearest TSS after 24 h of MENi in SEM and OCI-AML3 cells.
    Figure Legend Snippet: a , Heatmaps of Menin ChIP-seq at promoters and enhancers in SEM, RCH-ACV, and OCI-AML3 cells. Regions are sorted by H3K27ac signal and centered on ATAC peaks. b , Heatmaps of Menin ChIPmentation at promoters and enhancers, centred on H3K27ac peaks, in primary samples from two infant MLL-AF4 ALL patients (iALL28349, iALL863388) and one adult NPM1-mutated AML patient. c , Association between Menin binding at intragenic enhancers and differential gene expression from the nearest TSS following 24 h of MENi. p -values are reported from a Pearson chi-square test. d , Mean Menin, MLL/MLL-AF4, and H3K27ac at intragenic enhancers annotated to differentially expressed genes by nearest TSS after 24 h of MENi in SEM and OCI-AML3 cells.

    Techniques Used: ChIP-sequencing, Binding Assay, Gene Expression



    Similar Products

    rch acv  (DSMZ)
    96
    DSMZ rch acv
    a , Menin ChIP-seq after 24 h treatment with DMSO or 250 nM Menin inhibitor VTP50469 (MENi) in <t>SEM,</t> <t>RCH-ACV</t> and OCI-AML3 cells. Metaplots show mean reference normalized reads per million (RRPM) centered on transcription start sites (TSS) genome-wide. b , Dose response curves to MENi recorded at 48 h using CellTiterGlo (n = 5 for SEM and RCH-ACV cells, n = 3 for OCI-AML3 cells). c , Colony forming units recorded after culture in Menin inhibitor (n=5). Error bars represent standard error. d , Violin plots depicting the log2 fold changes of differentially expressed genes (FDR < 0.05) after 24 h MENi (250 nM), split by Menin binding at the promoter. e , Upset plot representing the cell lines in which shared Menin-bound genes (centre of Venn diagram in ) are downregulated following MENi (250 nM) for 24 h. f , Intersections of all upregulated genes following 24 h MENi (250 nM) in SEM, OCI-AML3, and RCH-ACV cells. g , Correlation heatmap of genome-wide Menin ChIP-seq signal in cell lines (SEM, RCH-ACV, and OCI-AML3) and Menin ChIPmentation in primary patient samples (NPM1c-AML, iALL28349, iALL863388). h , Heatmaps and metaplots showing the mean Menin signal at promoters in SEM cells (ChIP-seq) and two primary patient samples (ChIPmentation), sorted and k-means clustered by Menin signal in SEM cells. i , Heatmaps and metaplots showing the mean Menin signal at promoters in OCI-AML3 cells (ChIP-seq) and one primary patient sample (ChIPmentation), clustered by Menin signal in OCI-AML3 cells.
    Rch Acv, supplied by DSMZ, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rch+acv/bio_rxiv__64898__2026__01__16__698179-208-0-3?v=DSMZ
    Average 96 stars, based on 1 article reviews
    rch acv - by Bioz Stars, 2026-07
    96/100 stars
    		  Buy from Supplier
    kopn 8  (DSMZ)
    95
    DSMZ kopn 8
    a , Menin ChIP-seq after 24 h treatment with DMSO or 250 nM Menin inhibitor VTP50469 (MENi) in <t>SEM,</t> <t>RCH-ACV</t> and OCI-AML3 cells. Metaplots show mean reference normalized reads per million (RRPM) centered on transcription start sites (TSS) genome-wide. b , Dose response curves to MENi recorded at 48 h using CellTiterGlo (n = 5 for SEM and RCH-ACV cells, n = 3 for OCI-AML3 cells). c , Colony forming units recorded after culture in Menin inhibitor (n=5). Error bars represent standard error. d , Violin plots depicting the log2 fold changes of differentially expressed genes (FDR < 0.05) after 24 h MENi (250 nM), split by Menin binding at the promoter. e , Upset plot representing the cell lines in which shared Menin-bound genes (centre of Venn diagram in ) are downregulated following MENi (250 nM) for 24 h. f , Intersections of all upregulated genes following 24 h MENi (250 nM) in SEM, OCI-AML3, and RCH-ACV cells. g , Correlation heatmap of genome-wide Menin ChIP-seq signal in cell lines (SEM, RCH-ACV, and OCI-AML3) and Menin ChIPmentation in primary patient samples (NPM1c-AML, iALL28349, iALL863388). h , Heatmaps and metaplots showing the mean Menin signal at promoters in SEM cells (ChIP-seq) and two primary patient samples (ChIPmentation), sorted and k-means clustered by Menin signal in SEM cells. i , Heatmaps and metaplots showing the mean Menin signal at promoters in OCI-AML3 cells (ChIP-seq) and one primary patient sample (ChIPmentation), clustered by Menin signal in OCI-AML3 cells.
    Kopn 8, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rch+acv/pmc11874253-20-9-12?v=DSMZ
    Average 95 stars, based on 1 article reviews
    kopn 8 - by Bioz Stars, 2026-07
    95/100 stars
    		  Buy from Supplier
    dsmz acc 548  (DSMZ)
    95
    DSMZ dsmz acc 548
    a , Menin ChIP-seq after 24 h treatment with DMSO or 250 nM Menin inhibitor VTP50469 (MENi) in <t>SEM,</t> <t>RCH-ACV</t> and OCI-AML3 cells. Metaplots show mean reference normalized reads per million (RRPM) centered on transcription start sites (TSS) genome-wide. b , Dose response curves to MENi recorded at 48 h using CellTiterGlo (n = 5 for SEM and RCH-ACV cells, n = 3 for OCI-AML3 cells). c , Colony forming units recorded after culture in Menin inhibitor (n=5). Error bars represent standard error. d , Violin plots depicting the log2 fold changes of differentially expressed genes (FDR < 0.05) after 24 h MENi (250 nM), split by Menin binding at the promoter. e , Upset plot representing the cell lines in which shared Menin-bound genes (centre of Venn diagram in ) are downregulated following MENi (250 nM) for 24 h. f , Intersections of all upregulated genes following 24 h MENi (250 nM) in SEM, OCI-AML3, and RCH-ACV cells. g , Correlation heatmap of genome-wide Menin ChIP-seq signal in cell lines (SEM, RCH-ACV, and OCI-AML3) and Menin ChIPmentation in primary patient samples (NPM1c-AML, iALL28349, iALL863388). h , Heatmaps and metaplots showing the mean Menin signal at promoters in SEM cells (ChIP-seq) and two primary patient samples (ChIPmentation), sorted and k-means clustered by Menin signal in SEM cells. i , Heatmaps and metaplots showing the mean Menin signal at promoters in OCI-AML3 cells (ChIP-seq) and one primary patient sample (ChIPmentation), clustered by Menin signal in OCI-AML3 cells.
    Dsmz Acc 548, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rch+acv/pmc11871424-7-9-9?v=DSMZ
    Average 95 stars, based on 1 article reviews
    dsmz acc 548 - by Bioz Stars, 2026-07
    95/100 stars
    		  Buy from Supplier
    childhood b cell precursor acute lymphoblastic leukemia rch acv  (DSMZ)
    95
    DSMZ childhood b cell precursor acute lymphoblastic leukemia rch acv
    a , Menin ChIP-seq after 24 h treatment with DMSO or 250 nM Menin inhibitor VTP50469 (MENi) in <t>SEM,</t> <t>RCH-ACV</t> and OCI-AML3 cells. Metaplots show mean reference normalized reads per million (RRPM) centered on transcription start sites (TSS) genome-wide. b , Dose response curves to MENi recorded at 48 h using CellTiterGlo (n = 5 for SEM and RCH-ACV cells, n = 3 for OCI-AML3 cells). c , Colony forming units recorded after culture in Menin inhibitor (n=5). Error bars represent standard error. d , Violin plots depicting the log2 fold changes of differentially expressed genes (FDR < 0.05) after 24 h MENi (250 nM), split by Menin binding at the promoter. e , Upset plot representing the cell lines in which shared Menin-bound genes (centre of Venn diagram in ) are downregulated following MENi (250 nM) for 24 h. f , Intersections of all upregulated genes following 24 h MENi (250 nM) in SEM, OCI-AML3, and RCH-ACV cells. g , Correlation heatmap of genome-wide Menin ChIP-seq signal in cell lines (SEM, RCH-ACV, and OCI-AML3) and Menin ChIPmentation in primary patient samples (NPM1c-AML, iALL28349, iALL863388). h , Heatmaps and metaplots showing the mean Menin signal at promoters in SEM cells (ChIP-seq) and two primary patient samples (ChIPmentation), sorted and k-means clustered by Menin signal in SEM cells. i , Heatmaps and metaplots showing the mean Menin signal at promoters in OCI-AML3 cells (ChIP-seq) and one primary patient sample (ChIPmentation), clustered by Menin signal in OCI-AML3 cells.
    Childhood B Cell Precursor Acute Lymphoblastic Leukemia Rch Acv, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rch+acv/pmc11862126-270-1-20?v=DSMZ
    Average 95 stars, based on 1 article reviews
    childhood b cell precursor acute lymphoblastic leukemia rch acv - by Bioz Stars, 2026-07
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    a , Menin ChIP-seq after 24 h treatment with DMSO or 250 nM Menin inhibitor VTP50469 (MENi) in SEM, RCH-ACV and OCI-AML3 cells. Metaplots show mean reference normalized reads per million (RRPM) centered on transcription start sites (TSS) genome-wide. b , Dose response curves to MENi recorded at 48 h using CellTiterGlo (n = 5 for SEM and RCH-ACV cells, n = 3 for OCI-AML3 cells). c , Colony forming units recorded after culture in Menin inhibitor (n=5). Error bars represent standard error. d , Violin plots depicting the log2 fold changes of differentially expressed genes (FDR < 0.05) after 24 h MENi (250 nM), split by Menin binding at the promoter. e , Upset plot representing the cell lines in which shared Menin-bound genes (centre of Venn diagram in ) are downregulated following MENi (250 nM) for 24 h. f , Intersections of all upregulated genes following 24 h MENi (250 nM) in SEM, OCI-AML3, and RCH-ACV cells. g , Correlation heatmap of genome-wide Menin ChIP-seq signal in cell lines (SEM, RCH-ACV, and OCI-AML3) and Menin ChIPmentation in primary patient samples (NPM1c-AML, iALL28349, iALL863388). h , Heatmaps and metaplots showing the mean Menin signal at promoters in SEM cells (ChIP-seq) and two primary patient samples (ChIPmentation), sorted and k-means clustered by Menin signal in SEM cells. i , Heatmaps and metaplots showing the mean Menin signal at promoters in OCI-AML3 cells (ChIP-seq) and one primary patient sample (ChIPmentation), clustered by Menin signal in OCI-AML3 cells.

    Journal: bioRxiv

    Article Title: Menin maintains enhancer-promoter interactions in a leukemia-specific manner

    doi: 10.64898/2026.01.16.698179

    Figure Lengend Snippet: a , Menin ChIP-seq after 24 h treatment with DMSO or 250 nM Menin inhibitor VTP50469 (MENi) in SEM, RCH-ACV and OCI-AML3 cells. Metaplots show mean reference normalized reads per million (RRPM) centered on transcription start sites (TSS) genome-wide. b , Dose response curves to MENi recorded at 48 h using CellTiterGlo (n = 5 for SEM and RCH-ACV cells, n = 3 for OCI-AML3 cells). c , Colony forming units recorded after culture in Menin inhibitor (n=5). Error bars represent standard error. d , Violin plots depicting the log2 fold changes of differentially expressed genes (FDR < 0.05) after 24 h MENi (250 nM), split by Menin binding at the promoter. e , Upset plot representing the cell lines in which shared Menin-bound genes (centre of Venn diagram in ) are downregulated following MENi (250 nM) for 24 h. f , Intersections of all upregulated genes following 24 h MENi (250 nM) in SEM, OCI-AML3, and RCH-ACV cells. g , Correlation heatmap of genome-wide Menin ChIP-seq signal in cell lines (SEM, RCH-ACV, and OCI-AML3) and Menin ChIPmentation in primary patient samples (NPM1c-AML, iALL28349, iALL863388). h , Heatmaps and metaplots showing the mean Menin signal at promoters in SEM cells (ChIP-seq) and two primary patient samples (ChIPmentation), sorted and k-means clustered by Menin signal in SEM cells. i , Heatmaps and metaplots showing the mean Menin signal at promoters in OCI-AML3 cells (ChIP-seq) and one primary patient sample (ChIPmentation), clustered by Menin signal in OCI-AML3 cells.

    Article Snippet: RCH-ACV and OCI-AML3 (DSMZ) cells were grown in RPMI supplemented with 10% FBS and 1X GlutaMax (Gibco).

    Techniques: ChIP-sequencing, Genome Wide, Binding Assay

    a , Volcano plots of transient transcriptome RNA-seq after 24 h of treatment with DMSO or 250 nM Menin inhibitor VTP50469. b , Mean Menin binding (RRPM = reference normalised ChIP-seq reads per million) in SEM, RCH-ACV, and OCI-AML3 cells at promoters of differentially expressed genes in control (DMSO) or Menin inhibitor treatment (MENi). c , Proportion of differentially expressed genes (n.s. = not significant) following MENi with Menin binding at the promoter (± 2 kb around the TSS). Reported p -values are from a Pearson chi-square test. d , Intersections of all Menin-bound genes in the cell line panel. e , Intersections of downregulated Menin target genes in the cell line panel. f , Examples of Menin ChIP-seq in cell lines (SEM, RCH-ACV, and OCI-AML3) and ChIPmentation in primary patient samples (NPM1c-AML, iALL28349, and iALL863388) at down-regulated genes after 24 h MENi in the indicated cell lines.

    Journal: bioRxiv

    Article Title: Menin maintains enhancer-promoter interactions in a leukemia-specific manner

    doi: 10.64898/2026.01.16.698179

    Figure Lengend Snippet: a , Volcano plots of transient transcriptome RNA-seq after 24 h of treatment with DMSO or 250 nM Menin inhibitor VTP50469. b , Mean Menin binding (RRPM = reference normalised ChIP-seq reads per million) in SEM, RCH-ACV, and OCI-AML3 cells at promoters of differentially expressed genes in control (DMSO) or Menin inhibitor treatment (MENi). c , Proportion of differentially expressed genes (n.s. = not significant) following MENi with Menin binding at the promoter (± 2 kb around the TSS). Reported p -values are from a Pearson chi-square test. d , Intersections of all Menin-bound genes in the cell line panel. e , Intersections of downregulated Menin target genes in the cell line panel. f , Examples of Menin ChIP-seq in cell lines (SEM, RCH-ACV, and OCI-AML3) and ChIPmentation in primary patient samples (NPM1c-AML, iALL28349, and iALL863388) at down-regulated genes after 24 h MENi in the indicated cell lines.

    Article Snippet: RCH-ACV and OCI-AML3 (DSMZ) cells were grown in RPMI supplemented with 10% FBS and 1X GlutaMax (Gibco).

    Techniques: RNA Sequencing, Binding Assay, ChIP-sequencing, Control

    a , UpSet plot representing significantly enriched proteins (FDR < 0.01 compared to IgG controls) co-immunoprecipitated with Menin in SEM, OCI-AML3, and RCH-ACV cells. b , Heatmap of statistically enriched Menin interactors in SEM, RCH-ACV and OCI-AML3 cells. c , Global enrichment of select complexes involved in transcription in Menin co-immunoprecipitations. d , Correlation between the gene effect scores reported in DepMap for SEM and OCI-AML3 CRISPR screens. Proteins within complexes specifically enriched in SEM cells are colored. Genes with an effect score of < -1 in both cell lines are labelled.

    Journal: bioRxiv

    Article Title: Menin maintains enhancer-promoter interactions in a leukemia-specific manner

    doi: 10.64898/2026.01.16.698179

    Figure Lengend Snippet: a , UpSet plot representing significantly enriched proteins (FDR < 0.01 compared to IgG controls) co-immunoprecipitated with Menin in SEM, OCI-AML3, and RCH-ACV cells. b , Heatmap of statistically enriched Menin interactors in SEM, RCH-ACV and OCI-AML3 cells. c , Global enrichment of select complexes involved in transcription in Menin co-immunoprecipitations. d , Correlation between the gene effect scores reported in DepMap for SEM and OCI-AML3 CRISPR screens. Proteins within complexes specifically enriched in SEM cells are colored. Genes with an effect score of < -1 in both cell lines are labelled.

    Article Snippet: RCH-ACV and OCI-AML3 (DSMZ) cells were grown in RPMI supplemented with 10% FBS and 1X GlutaMax (Gibco).

    Techniques: Immunoprecipitation, CRISPR

    a , Pearson correlations between nuclear proteome samples (left) and Menin immunoprecipitation (IP) samples (right), computed on log2-transofrmed and imputed data. b , Correlation in the abundance of differentially enriched complex components in the nuclear proteomes of SEM and OCI-AML3 cells. The grey dashed line represents a slope of 1. Pearson R values are shown for each complex. c , Comparison of proteins enriched in Menin IPs in SEM versus RCH-ACV cells (left) and SEM versus OCI-AML3 cells (right). Only proteins that were statistically enriched over IgG controls in at least one cell line (FDR < 0.01) were included.

    Journal: bioRxiv

    Article Title: Menin maintains enhancer-promoter interactions in a leukemia-specific manner

    doi: 10.64898/2026.01.16.698179

    Figure Lengend Snippet: a , Pearson correlations between nuclear proteome samples (left) and Menin immunoprecipitation (IP) samples (right), computed on log2-transofrmed and imputed data. b , Correlation in the abundance of differentially enriched complex components in the nuclear proteomes of SEM and OCI-AML3 cells. The grey dashed line represents a slope of 1. Pearson R values are shown for each complex. c , Comparison of proteins enriched in Menin IPs in SEM versus RCH-ACV cells (left) and SEM versus OCI-AML3 cells (right). Only proteins that were statistically enriched over IgG controls in at least one cell line (FDR < 0.01) were included.

    Article Snippet: RCH-ACV and OCI-AML3 (DSMZ) cells were grown in RPMI supplemented with 10% FBS and 1X GlutaMax (Gibco).

    Techniques: Immunoprecipitation, Comparison

    a , Heatmaps of Menin ChIP-seq at promoters and enhancers in SEM, RCH-ACV, and OCI-AML3 cells. Regions are sorted by H3K27ac signal and centered on ATAC peaks. b , Heatmaps of Menin ChIPmentation at promoters and enhancers, centred on H3K27ac peaks, in primary samples from two infant MLL-AF4 ALL patients (iALL28349, iALL863388) and one adult NPM1-mutated AML patient. c , Association between Menin binding at intragenic enhancers and differential gene expression from the nearest TSS following 24 h of MENi. p -values are reported from a Pearson chi-square test. d , Mean Menin, MLL/MLL-AF4, and H3K27ac at intragenic enhancers annotated to differentially expressed genes by nearest TSS after 24 h of MENi in SEM and OCI-AML3 cells.

    Journal: bioRxiv

    Article Title: Menin maintains enhancer-promoter interactions in a leukemia-specific manner

    doi: 10.64898/2026.01.16.698179

    Figure Lengend Snippet: a , Heatmaps of Menin ChIP-seq at promoters and enhancers in SEM, RCH-ACV, and OCI-AML3 cells. Regions are sorted by H3K27ac signal and centered on ATAC peaks. b , Heatmaps of Menin ChIPmentation at promoters and enhancers, centred on H3K27ac peaks, in primary samples from two infant MLL-AF4 ALL patients (iALL28349, iALL863388) and one adult NPM1-mutated AML patient. c , Association between Menin binding at intragenic enhancers and differential gene expression from the nearest TSS following 24 h of MENi. p -values are reported from a Pearson chi-square test. d , Mean Menin, MLL/MLL-AF4, and H3K27ac at intragenic enhancers annotated to differentially expressed genes by nearest TSS after 24 h of MENi in SEM and OCI-AML3 cells.

    Article Snippet: RCH-ACV and OCI-AML3 (DSMZ) cells were grown in RPMI supplemented with 10% FBS and 1X GlutaMax (Gibco).

    Techniques: ChIP-sequencing, Binding Assay, Gene Expression